本文采用标准加入法,在237nm 和278.5nm 波长处测定吸收度,以系数倍率法消除人血浆和硝苯吡啶体外降解产物紫外吸收光谱的相互干扰,从而直接测定了人血浆硝笨吡啶的含量。血浆(沉淀蛋白质后的)加样回收率为100.7%,CV=0.74%(n=8)。本法具有操作简便、快速和结果准确可靠等优点。 In this paper, the standard addition method was used to determine the absorbance at 237 nm and 278.5 nm wavelength, and the coefficient of multiples method was used to eliminate the interference of ultraviolet absorption spectra of human plasma and nifedipine degradation products in vitro to directly determine the nitrobenzopyridine content in human plasma. The plasma sample recovery (after protein precipitation) was 100.7% with CV = 0.74% (n = 8). This method has the advantages of easy operation, fast and accurate and reliable results.