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目的 :探索肌肉来源的细胞是否能分化为造血细胞 ,为成体干细胞具有可塑性的新观点寻找依据。方法 :通过体外造血集落培养 ,对比观察肌肉来源细胞和骨髓有核细胞的CFU_GM ,CFU_GEMM和BFU_E的形成率 ;利用小鼠细胞移植模型 ,以及流式细胞仪细胞纯化技术 ,长期观察未纯化的、Ly5 .2 + 和Ly5 .2 _肌肉来源细胞的造血潜能。结果 :体外培养 ,骨髓有核细胞的CFU_GM ,CFU_GEMM和BFU_E的形成率分别为 (1 6 0 .3± 1 7.5 ) / 1 0 5,(8.5±1 .8) / 1 0 5和 (1 8.8± 2 .5 ) / 1 0 5,而肌肉来源细胞的CFU_GM的形成率为 (1 0 8.3± 4 2 .5 ) / 1 0 5,未见CFU_GEMM和BFU_E集落形成。体内实验 ,在未纯化的肌肉来源细胞组 ,肌肉来源细胞的造血能力低于造血细胞的 1 0 % ,而且此能力观察 1 2个月无下降 ;分选后 ,无论来源于新鲜的 (未培养 )或培养后的肌肉来源细胞 ,在Ly5 .2 + 细胞组 ,同样可检测到其造血潜能 ,而在Ly5 .2 _细胞组未检测到。结论 :小鼠肌肉来源的细胞具有造血潜能 ,具有此功能的干细胞为髓源性的 ,而非肌肉干细胞横向分化所致 ;是否存在有既能分化为肌肉细胞 ,又能分化为造血细胞的多潜能干细胞 ,还有待进一步的研究
Objective: To explore whether muscle-derived cells can differentiate into hematopoietic cells and find the basis for new ideas of adult stem cells with plasticity. METHODS: The CFU-GM, CFU-GMEM and BFU-E formation rates of muscle-derived cells and bone marrow-derived nucleated cells were compared by hematopoietic cell culture in vitro. The unpurified, Hematopoietic potential of Ly5 .2 + and Ly5. 2 _ muscle-derived cells. Results: The rates of CFU_GM, CFU_GEMM and BFU_E in bone marrow nucleated cells were (160.3 ± 7.5) / 105, (8.5 ± 1.8) / 105 and ± 2 .5) / 1 0 5, while the CFU-GM formation rate of muscle-derived cells was (1 0 8.3 ± 4 2 .5) / 1 0 5, no CFU_GEMM and BFU_E colony formation were observed. In vivo experiments showed that the hematopoietic capacity of muscle-derived cells was lower than 10% of that of hematopoietic cells in the unpurified muscle-derived cell group, and there was no decrease in the ability to observe for 12 months. After sorting, both cells derived from fresh ) Or cultured muscle-derived cells in the Ly5 .2 + cell group, the same hematopoietic potential can be detected, but not in Ly5 .2_ cell group. CONCLUSIONS: The muscle-derived cells in mice have the hematopoietic potential. The stem cells with this function are of myeloid origin, not caused by the lateral differentiation of muscle stem cells. Whether there are many cells that can differentiate into muscle cells and hematopoietic cells Potential stem cells, remains to be further studied