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目的探讨转染次级淋巴趋化因子基因的树突细胞(DC)瘤苗在小鼠前列腺癌中的抗肿瘤效应。方法通过脂质体法将次级淋巴趋化因子基因转染至小鼠骨髓来源的树突状细胞,构建 DC 瘤苗;逆转录-聚合酶链反应检测 SLC;种瘤24 d 后 SLC 组的瘤体平均体积为(1430±86)mm~3,45 d 后仍有50%的荷瘤小鼠存活,与其他两组比较差异有统计学意义(P<0.05);免疫组织化学荧光染色可见 SLD 组中 CD4~+、CD8~+T 细胞和 CD11~+DC 的浸润率分别为(15.24±0.67)%、(11.02±0.73)%和(14.50±0.51)%,与 DC 组比较差异有统计学意义(P<0.05)。结论转染SLC 的 DC 瘤苗能有效诱导抗肿瘤的免疫效应,其免疫效应是通过趋化大量 CD4~+、CD8~+T 细胞及CD11~+DC 至肿瘤部位而发挥作用。
Objective To investigate the anti-tumor effect of dendritic cell (DC) vaccine transfected with secondary lymphokine-chemokine gene in mouse prostate cancer. Methods Lipopolysaccharide was transfected into mouse bone marrow-derived dendritic cells to construct DC vaccine. SLC was detected by reverse transcription-polymerase chain reaction 50% tumor-bearing mice survived after an average volume of 1430 ± 86 mm ~ 3,45 d, which was significantly different from the other two groups (P <0.05). Immunohistochemical staining revealed The infiltration rates of CD4 ~ +, CD8 ~ + T cells and CD11 ~ + DC in SLD group were (15.24 ± 0.67)%, (11.02 ± 0.73)% and (14.50 ± 0.51)%, respectively Significance (P <0.05). CONCLUSION: DC vaccine transfected with SLC can effectively induce the anti-tumor immune effect. The immune effect is mediated through the chemotaxis of a large number of CD4 ~ +, CD8 ~ + T cells and CD11 ~ + DC to the tumor site.