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目的研究来曲唑与二甲双胍治疗子宫内膜异位症的作用。方法采用本体子宫移植的方法,人为诱发大鼠子宫内膜异位症,并检测来曲唑与二甲双胍治疗前后病灶部位体积的变化以及雌性激素合成相关蛋白的表达。36例子宫内膜异位症模型鼠随机分为来曲唑组和二甲双胍组,并提取子宫内膜在位内膜细胞进行体外培养,观察不同浓度的来曲唑和二甲双胍对体外培养细胞的增殖能力和细胞凋亡的影响。采用MTT法检测细胞增殖能力的变化,使用流式细胞术分析细胞周期分布和细胞凋亡情况。结果用来曲唑和二甲双胍处理后,病灶部位有明显的减小,雌激素相关蛋白的表达受到抑制,体外培养细胞的增殖能力、细胞周期、细胞凋亡都受到影响。来曲唑对雌激素相关蛋白的影响优于二甲双胍;而二甲双胍对于细胞周期的影响优于来曲唑。结论来曲唑与二甲双胍对子宫内膜异位症均有治疗效果。
Objective To study the effect of letrozole and metformin in the treatment of endometriosis. Methods The method of ontology uterine transplantation was used to induce endometriosis in rats. The changes of lesion volume and the expression of estrogen related proteins were detected before and after treatment with letrozole and metformin. 36 cases of endometriosis model mice were randomly divided into letrozole group and metformin group, and the endometrial cells in endometriosis were extracted for in vitro culture. The effects of different concentrations of letrozole and metformin on the proliferation of cultured cells in vitro Effects and Apoptosis. Cell proliferation was measured by MTT assay. Cell cycle distribution and apoptosis were analyzed by flow cytometry. Results After treated with letrozole and metformin, the lesions were significantly reduced and the expression of estrogen related proteins was inhibited. The proliferation, cell cycle and apoptosis of cultured cells were affected. Letrozole had a greater effect on estrogen-related proteins than metformin, whereas metformin had better effects on cell cycle than letrozole. Conclusion Letrozole and metformin have therapeutic effects on endometriosis.