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目的 :研究大鼠局灶性脑缺血后血管内皮细胞生长因子 (VEGF)表达的动态变化。方法 :采用尼龙线栓法制作大鼠局灶性永久性大脑中动脉闭塞模型 ,用免疫组织化学方法观察大鼠缺血后 3、6、12h和 1、2、3、7d时 ,VEGF表达的情况。结果 :大鼠缺血后 3h ,缺血侧脑组织开始出现VEGF表达 ,2 4h明显增多 ,2d达高峰 ,7d时仍有少量表达。各时间占VEGF的表达主要集中在梗死灶周围。结论 :VEGF可能参与缺血性脑损伤的病理发展及修复过程
Objective: To investigate the dynamic changes of vascular endothelial growth factor (VEGF) expression after focal cerebral ischemia in rats. Methods: The model of focal middle cerebral artery occlusion (MCAO) was established by nylon thread plug method. The expression of VEGF at 3, 6, 12 hours and 1, 2, 3 and 7 days after ischemia was observed by immunohistochemistry Happening. Results: 3h after ischemia, the expression of VEGF began to appear in the ischemic brain tissue, significantly increased at 24 h and reached the peak at 2 d, with little expression at 7 d. The expression of VEGF in each time mainly concentrated around the infarct. Conclusion: VEGF may be involved in the pathological development and repair process of ischemic brain injury