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目的:探讨当归多糖(APS)、大黄多糖(RTP)与腹腔巨噬细胞(Mφ)甘露糖受体(MR)的结合特性及其对腹腔Mφ免疫功能的影响。方法:以异硫氰酸荧光素标记的甘露糖化牛血清白蛋白(Man-FITC-BSA)可特异性的与甘露糖受体相结合为模型,以甘露糖为阳性对照、半乳糖为阴性对照,通过竞争性抑制实验,将APS、RTP分别与腹腔Mφ共同孵育60min后,用荧光显微镜和多功能酶标仪检测腹腔Mφ摄取Man-FITC-BSA的情况;通过ELISA方法检测APS、RTP刺激Mφ分泌TNF-α及IL-4的情况。结果:D-甘露糖、APS、RTP均可抑制腹腔Mφ吞噬Man-FITC-BSA,而D-半乳糖则没有此作用;APS、RTP均可诱导腹腔Mφ分泌TNF-α,且呈剂量依赖性,甘露糖可完全阻滞RTP诱导Mφ分泌TNF-α,对APS诱导Mφ分泌TNF-α只有部分对抗作用;两种多糖对IL-4的分泌没有统计学意义;甘露糖不能诱导Mφ分泌TNF-α与IL-4。结论:RTP引起的Mφ分泌TNF-α作用是通过甘露糖受体介导,而APS的作用不仅仅与甘露糖受体有关。两种多糖与甘露糖受体的作用差异可能与其单糖组成密切相关。
Objective: To investigate the binding properties of angelica polysaccharide (APS), rhubarb polysaccharide (RTP) and mannose receptor (MR) in peritoneal macrophages (Mφ) and its effect on the immune function of abdominal cavity Mφ. Methods:Man-FITC-BSA labeled with fluorescein isothiocyanate can be specifically combined with mannose receptor as a model, mannose as a positive control and galactose as a negative control. After competitive inhibition experiments, APS and RTP were incubated with peritoneal Mφ for 60 min. Fluorescence microscopy and a multi-functional microplate reader were used to detect the uptake of Man-FITC-BSA in peritoneal Mφ; APS and RTP stimulation were measured by ELISA. Secretion of TNF-α and IL-4. RESULTS: D-mannose, APS and RTP all inhibited peritoneal Mφ phagocytosis of Man-FITC-BSA, while D-galactose did not. APS and RTP could induce TNF-α secretion in peritoneal Mφ in a dose-dependent manner. , Mannose can completely block the RTP-induced Mφ secretion of TNF-α, only partially counteract the secretion of TNF-α induced by APS induced Mφ; two polysaccharides have no statistical significance for the secretion of IL-4; mannose can not induce Mφ secretion TNF- α and IL-4. CONCLUSION: The effect of RTP-induced secretion of TNF-α by Mφ is mediated by the mannose receptor, and the role of APS is not only related to the mannose receptor. The difference in the action of two polysaccharides and mannose receptors may be closely related to their monosaccharide composition.