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目的:研究厌食症幼鼠胃窦Cajal间质细胞的损伤与健儿散对其的修复作用,从而探讨健儿散干预厌食症的可能机制。方法:40只SPF级SD大鼠幼鼠随机分为模型对照组、正常对照组、健胃消食片对照组和健儿散干预组,正常对照组给予正常饮食,其余3组采用特制饲料喂养法造小儿厌食症动物模型。造模成功后,分别灌胃生理盐水、生理盐水、江中健胃消食片混悬液和健儿散溶液,连续给药4周。观察记录各组大鼠摄食量和体质量的变化,采用透射电子显微镜、免疫组织化学法及RT-PCR法检测各组大鼠胃窦Cajal间质细胞的超微结构、C-kit阳性ICC的数量与分布以及C-kit mRNA的基因表达水平。结果:与模型对照组相比,健儿散干预组的平均摄食量与平均体质量均明显增加(P<0.05),胃窦ICC超微结构的损伤明显修复和改善,Cajal间质细胞c-kit阳性ICC数量显著增加(P<0.05),胃窦C-kit mRNA的基因表达水平显著增加(P<0.05)。结论:健儿散能够增加厌食症幼鼠的摄食量与体质量,修复ICC超微结构的损伤,增加胃窦ICC的表达数量,提高C-kit mRNA基因的表达水平,改善ICC功能并调节其分布,从而达到促进胃肠动力的作用。
Objective: To study the injury of Cajal interstitial cells in antrum of anorexia rats and the repair effect of Jianliansan on it, so as to explore the possible mechanism of bradyrhizobia in the treatment of anorexia. Methods: Forty SPF SD rats were randomly divided into normal control group, normal control group, Jianweixiaoshi tablet control group, Jianfu Sangan group and normal control group, and the other three groups were given special feeding method Pediatric anorexia animal model. After the success of modeling, respectively, gavage saline, saline, Jiangzhongwei Wei Xiaobian suspension and Jian San scattered solution, continuous administration for 4 weeks. The changes of food intake and body weight were observed and recorded. The ultrastructure of Cajal interstitial cells in gastric antrum were detected by transmission electron microscopy, immunohistochemistry and RT-PCR. The positive rates of C-kit positive ICC Quantity and distribution, and gene expression level of C-kit mRNA. Results: Compared with the model control group, the average food intake and the average body weight of the intervention group were significantly increased (P <0.05), and the damage of ICC ultrastructure in the antrum was significantly repaired and improved. The c-kit of Cajal interstitial cells The number of positive ICC was significantly increased (P <0.05), and the gene expression of C-kit mRNA in gastric antrum was significantly increased (P <0.05). Conclusion: Jianliansan can increase food intake and body weight of juvenile rats with anorexia, repair the damage of ICC ultrastructure, increase the expression of ICC in gastric antrum, increase the expression of C-kit mRNA, improve the function of ICC and regulate its distribution , So as to achieve the role of gastrointestinal motility.