流式细胞术(Flow cytometry简称FCM)是近年来发展的一种快速定量分析细胞的新技术,它具有快速、精确和重复性好等特点。在国外已广泛用于肿瘤研究和医学基础研究在此技术中,单细胞悬液的制备是关键环节,尤其是实体肿瘤组织的单细胞悬液制备甚为重 Flow cytometry (FCM) is a new technique for the rapid quantitative analysis of cells that has been developed in recent years. It has features such as rapidity, precision, and good repeatability. It has been widely used in cancer research and basic medical research in foreign countries. In this technology, the preparation of single cell suspensions is a key step, especially for the preparation of single cell suspensions in solid tumor tissues.