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目的:建立小儿惊风散中甘草酸含量的测定方法。方法:采用HPLC法,色谱柱:YMC-Pack ODS C_(18)柱(250 mm×4.6 mm,5μm)。流动相:乙腈-0.1 mol·L~(-1)醋酸铵溶液-冰醋酸(28:72:0.3);流速为1.0ml·min~(-1);检测波长为250 nm;温度:35℃。结果:甘草酸的线性范围是0.58～2.91μg,r=0.999 5。平均加样回收率99.4%,RSD=1.5%(n=9)。结论:该法可以用于测定小儿惊风散中甘草酸的含量。
Objective: To establish a method for the determination of glycyrrhizic acid in children’s Jingfeng Powder. Method: HPLC method was used. Column: YMC-Pack ODS C 18 column (250 mm×4.6 mm, 5 μm). Mobile phase: acetonitrile-0.1 mol·L -1 ammonium acetate solution-glacial acetic acid (28:72:0.3); flow rate 1.0 ml·min -1; detection wavelength at 250 nm; temperature: 35°C . Results: The linear range of glycyrrhizic acid was 0.58-2.91 μg, r=0.999 5. The average recovery rate was 99.4% with RSD=1.5% (n=9). Conclusion: This method can be used to determine the content of glycyrrhizic acid in children’s Jingfeng Powder.