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Hca-F25/CL-16A_3(以下简称16A_3)和Hca-F25/CL-A_2(以下简称A_2)是两个来自共同祖先Hca-F25/L的具特异性淋巴道转移能力的小鼠腹水型肝癌细胞系。转移率前者为78.6～89.4％,后者为15～15.4％。通过细胞遗传学某些指标的观察,我们发现两系细胞存在着差异。核型分析发现,A_2系众数为42,标记染色体有四种,各一条;16A_3系众数为81和84,标记染色体也有四种,各双份,显带带型与A_2系之标记染色体完全相同。对银染核仁组织者区的观察发现:问期细胞的Ag-NORs数平均为:A_2 44个、16A_368个;中期细胞Ag-NORs平均数为:A_28.4个、16A_3 13.8个,高转移力的16_3A细胞明显高于低转移力的A_2细胞。结果提示:16A_3细胞可能是在祖先群体中由A_2细胞经核内复制而来;一些基因(如γDNA)的倍增和活性表达与16A_3高转移能力的形成可能有关。
Hca-F25/CL-16A_3 (hereinafter referred to as 16A_3) and Hca-F25/CL-A_2 (abbreviated as A_2 hereinafter) are two mouse ascitic liver cancers with specific lymphatic metastasis ability from the common ancestor Hca-F25/L. Cell line. The former was 78.6 to 89.4%, and the latter was 15 to 15.4%. Through the observation of some indicators of cytogenetics, we found that there are differences between the two lines of cells. The karyotype analysis revealed that the A2 family has a mode of 42, and there are four marker chromosomes, one each; the 16A-3 family has 81 and 84 modes, and there are also four marker chromosomes, each of which has a double band, a banding pattern and a marker chromosome of the A2 line. Exactly the same. The silver-stained nucleolar organizer observed that the number of Ag-NORs in the patient’s cells during the interview period was 44 in average and 16A-368 in the A_2; the average number of Ag-NORs in the metaphase cells was: A_28.4, and 16A_3 13.8. The force of 16_3A cells was significantly higher than that of low metastatic A_2 cells. The results suggest that 16A_3 cells may have been nucleus-derived from A_2 cells in the ancestral population; the doubling and active expression of some genes (such as γDNA) may be related to the formation of high metastatic potential of 16A_3.