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目的:应用聚合酶链反应(PCR)检测细菌16SrRNA基因,研究其在新生儿败血症中的应用。方法:抽取临床疑为细菌感染的265例新生儿的静脉血,分别做血培养和细菌16SrRNA的基因检测。于血标本中抽提DNA后用聚合酶链反应(PCR)扩增,将扩增产物电泳后经凝胶成像系统扫描。结果:PCR检测阳性率为6.4%(17/265),明显高于血培养的阳性率〔3.4%(9/265)〕,差异有统计学意义(P<0.05)。结论:与血培养相比,检测细菌16SrRNA基因对新生儿败血症诊断的特异性和敏感度更高。
Objective: To detect the bacterial 16SrRNA gene by polymerase chain reaction (PCR) and study its application in neonatal sepsis. Methods: Venous blood from 265 newborns suspected of bacterial infection was collected and tested for blood culture and bacterial 16SrRNA respectively. DNA was extracted from the blood sample and amplified by polymerase chain reaction (PCR). The amplified product was electrophoresed and scanned by a gel imaging system. Results: The positive rate of PCR detection was 6.4% (17/265), which was significantly higher than that of blood culture [3.4% (9/265)]. The difference was statistically significant (P <0.05). Conclusion: Compared with blood culture, detection of bacterial 16S rRNA gene is more specific and sensitive in the diagnosis of neonatal sepsis.