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目的调查韶关市分散式供水中大肠埃希菌的污染及其耐药情况,及时发现水性传染源,为保障公共卫生安全提供科学依据。方法采集市内山泉水、井水、储水池水、水库水和溪(河)水等5种水样共计120份,依据GB/T5750-2006《生活饮用水标准检验方法》进行菌落总数、总大肠菌群、耐热大肠菌群、大肠埃希菌4个微生物项目的检测,对检出的大肠埃希菌进行抗生素耐药性检测,并对实验结果进行统计分析。结果 120份水样中有42份微生物学指标均合格,总合格率为35.0%,检出大肠埃希菌42株,其中7株为致腹泻大肠埃希菌。85.7%的大肠埃希菌对21种抗生素的一种或几种具有耐药性,42.9%的菌株呈多重耐药性,5株为产ESBLs大肠埃希菌。结论韶关市分散式供水的水质情况不容乐观,不同水源中存在不同比例的致病性和耐药性大肠埃希菌,市民应谨慎选择饮用水源,饮用前应消毒处理。相关部门应加强管理和提供健康教育,确保居民饮水安全。
Objective To investigate the pollution and drug resistance of Escherichia coli in distributed water supply in Shaoguan City and find out the source of waterborne infection in time to provide a scientific basis for the protection of public health. Methods A total of 120 samples were collected from five kinds of spring water, well water, reservoir water, reservoir water and river (river) water in the city. According to GB / T5750-2006 Standard Test Method for Domestic Drinking Water, the total number of colonies, Bacteria, heat-resistant coliform bacteria, Escherichia coli four microbial tests, the detection of E. coli antibiotic resistance test, and the experimental results were statistically analyzed. Results 42 of the 120 water samples were qualified for microbiology, with a total pass rate of 35.0%. 42 strains of Escherichia coli were detected, of which 7 were Escherichia coli causing diarrhea. 85.7% of Escherichia coli strains were resistant to one or more of the 21 antibiotics, 42.9% of the isolates were multi-drug resistant, and 5 were ESBLs-producing Escherichia coli. Conclusion The water quality of decentralized water supply in Shaoguan City is not optimistic. Different pathogenic and drug-resistant Escherichia coli strains exist in different water sources. Citizens should choose source of drinking water with caution and disinfect before drinking. Relevant departments should strengthen management and provide health education to ensure that residents drinking water safety.