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目的 研究严重急性呼吸综合征(SARS)患者感染后体内病毒特异性抗体产生规律。方法 收集临床确诊为SARS患者的血清和非SARS人群血清标本,用IgM捕获法、间接法和抗原夹心法三种不同方法检测抗SARS病毒特异性IgM、IgG和总抗体。结果 检测146份临床诊断为SARS的患者不同发病时间血清标本,三种抗体阳性率分别为61.64％、53.43％和69.86％;SARS病毒特异性IgM、IgG抗体的最早检出时间分别在发病第7天和第12天,特异性IgM抗体最短在发病后42天消失。三种方法检测70份甲型肝炎患者血清时,均有2份非特异阳性反应,检测127份其他病种血清均阴性,1例密切接触SARS患者的医务人员SARS特异性IgG抗体和总抗体均阳性,三种检测方法均不受类风湿因子影响。结论 与其他病毒感染相比,SARS病毒感染者的特异性IgM抗体检出时间较晚,且持续时间较短;三种检测方法均有较好的特异性和敏感性,可用于SARS的流行病学调查和临床诊断的确认和补充,但不适用于SARS的早期诊断。
Objective To study the rule of virus-specific antibody production in patients with severe acute respiratory syndrome (SARS) infection. Methods Serum samples from SARS patients and non-SARS patients were collected. IgM, IgG and total antibodies against SARS virus were detected by IgM capture method, indirect method and antigen sandwich method. Results The serum samples of 146 patients with clinically diagnosed SARS at different times of onset were detected. The positive rates of the three antibodies were 61.64%, 53.43% and 69.86%, respectively. The earliest detection time of SARS-specific IgM and IgG was 7 On days 12 and 12, the shortest specific IgM antibody disappeared 42 days after onset. There were 2 nonspecific positive reactions in 70 samples of hepatitis A patients detected by the three methods. The serums of 127 other patients were negative. The SARS-specific IgG antibodies and total antibodies of 1 SARS-infected SARS patients Positive, the three detection methods are not affected by rheumatoid factor. Conclusion Compared with other virus infections, the specific IgM antibodies detected by SARS virus are detected later and have a shorter duration. All three detection methods have good specificity and sensitivity and can be used for the epidemic of SARS The investigation and clinical diagnosis of confirmation and supplement, but not for the early diagnosis of SARS.