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羊草是我国北方草原群落重要的建群种和优势种。本研究应用AFLP(扩增片段长度多态性)的方法研究了4个羊草自然群落的遗传结构。应用8个引物组合,共得到489个AFLP带,从85到550 bp,结果表明,1)4个群落的遗传多样性和平均多态条带百分率有相似的趋势,即W(锡林浩特)>H(海拉尔)>D(大庆)>J(长岭)。2)用非加权配对算数平均法(UPGMA)聚类分析的结果表明,同一种群的个体亲缘关系近,而在不同种群之间经向基因流较纬向基因流更为频繁。探讨了形成这种现象的原因,即风向和候鸟在种群间基因流中的作用。
Leymus chinensis is an important species and dominant species of grassland community in northern China. In this study, AFLP (amplified fragment length polymorphism) method was used to study the genetic structure of four natural populations of Leymus chinensis. A total of 489 AFLP bands were obtained from 85 to 550 bp using 8 primer combinations. The results showed that there was a similar tendency among 1) the genetic diversity and average percentage of polymorphic bands in 4 communities, ie W (Xilinhot)> H (Hailar)> D (Daqing)> J (Changling). 2) The result of UPGMA clustering analysis showed that the individuals of the same population had close genetic relationship, while the warp gene flow was more frequent between different populations than the zonal flow. The reasons for this phenomenon were discussed, namely the direction of the wind and the role of migratory birds in gene flow between populations.