棉花的远缘杂交对于培育新品种、创造雄性不育系、探索棉属的分类和进化都有重要作用。目前,有的国家(如苏、美)已把种间、属间和地理远缘种内杂交工作放到棉花育种的首要地位。然而由于种间杂交存在着不亲和性,即主要表现为杂种胚乳和胚的早期败育,因而很难获得杂种。五十年代就有不少研究者提出体外培养杂种胚珠,有可能克服棉属种间杂交的不亲和性。Stewast(1978)体外培养了四种栽培棉之间的八个正反交的杂种胚珠,结果除A_1×AD_2外,其余组合在不同程度上都获得了杂种植株,有的组合杂种成苗率达培养胚珠的40％。但对培养的有关细节,也没有详细报导。为此我们培养了AD_1×A_2的杂种胚珠,并且在母体杂交花朵上混喷赤霉素和萘 The distant hybridization of cotton plays an important role in cultivating new varieties, creating male sterile lines and exploring the classification and evolution of cotton genus. At present, some countries (such as the Soviet Union and the United States) have placed the cross-species, inter-genus and geographical distant hybridization work at the top of cotton breeding. However, it is difficult to obtain hybrids due to the incompatibility of interspecific hybrids, ie, the early appearance of hybrid endosperm and embryos. In the 1950s, many researchers proposed that in vitro culture of hybrid ovules may overcome the incompatibility of cotton hybrids. Stewast (1978) cultured eight cross between the four cultivated cotton hybrid ovules, the results except A_1 × AD_2, the rest of the combinations to varying degrees, all get hybrid plants, and some combinations of hybrid seedling rate up to Ovule culture 40%. However, there is no detailed report on the details of training. To this end, we cultivated the hybrid ovules of AD_1 × A_2, and mixed the gibberellin and naphthalene