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目的探讨 ＣＤ４４Ｖ６蛋白和ＴＧＦ β在喉癌中的表达与转移及预后的关系。方法用 ＣＤ４４Ｖ６蛋白和 ＴＧＦ β单克隆抗体，通过免疫组化Ｓ－Ｐ法，对６９例喉鳞状细胞癌蜡块标本和２０例正常喉粘膜标本进行研究，结合临床及随访资料进行分析。结果６９例喉鳞状细胞癌的 ＣＤ４４Ｖ６蛋白和 ＴＧＦ β阳性表达率分别为 ５２％（３６／６９），７７％（５３／６９）。２０例正常喉粘膜ＣＤ４４Ｖ６蛋白和ＴＧＦ β阳性表达率为０（０／２０）．ＣＤ４４Ｖ６蛋白和ＴＧＦβ阳性表达与肿瘤原发部位、组织分级和临床分期无相关性（Ｐ＞０．０５）；颈部淋巴结转移的病例原发瘤的ＣＤ４４Ｖ６蛋白和ＴＧＦβ阳性表达分别为６０％（２４／４０），６５％（２６／４０），无淋巴结转移的病例ＣＤ４４Ｖ６蛋白和ＴＧＦβ阳性表达率分别为２８％（８／２９），３４％（１０／２９），差别有显著意义。用 ｌｏｇ－ ｒａｎｋ检验分析 ＣＤ４４Ｖ６蛋白和 ＴＧＦ β表达，阴性组 ５ ａ生存率高于阳性表达组（Ｐ＜ ０．０５）。结论喉鳞癌 ＣＤ４４Ｖ６蛋白和 ＴＧＦ β的阳性表达与颈部淋巴结转移和 ５ ａ生存率显著相关， ＣＤ４Ｖ６蛋白和ＴＧＦβ可作为预测喉癌转移和预后的重要指标。
Objective To investigate the relationship between the expression of CD44V6 protein and TGF β in laryngeal carcinoma and metastasis and prognosis. Methods Totally 69 specimens of laryngeal squamous cell carcinoma and 20 normal laryngeal mucosa specimens were studied by immunohistochemical S-P method using CD44V6 protein and TGF β monoclonal antibody. The clinical and follow-up data were analyzed. Results The positive rates of CD44V6 and TGF β in 69 cases of laryngeal squamous cell carcinoma were 52% (36/69) and 77% (53/69), respectively. The positive expression rates of CD44V6 protein and TGF β in 20 cases of normal laryngeal mucosa were 0 (0/20). The positive expression of CD44V6 protein and TGFβ had no correlation with the primary tumor location, histological grade and clinical stage (P> 0.05). The positive expression rates of CD44V6 protein and TGFβ in the primary tumor with cervical lymph node metastasis were 60% (24% / 40), 65% (26/40) respectively. The positive rates of CD44V6 and TGFβ in cases without lymph node metastasis were 28% (8/29) and 34% (10/29), respectively. Log-rank test was used to analyze the expression of CD44V6 protein and TGFβ. The 5-year survival rate of the negative group was higher than that of the positive group (P <0.05). Conclusion The positive expression of CD44V6 protein and TGFβ in LSCC is significantly correlated with lymph node metastasis and 5-year survival rate of laryngeal squamous cell carcinoma. CD4V6 protein and TGFβ may be used as an important index in predicting the metastasis and prognosis of LSCC.