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目的:建立藿香正气水中橙皮苷的含量测定方法。方法:高效液相色谱法测定藿香正气水中橙皮苷的含量。选用 Hypersil ODS C-18反相色谱柱;以乙腈-8％醋酸溶液(10:90)为流动相;检测波长284 nm,流速1．0 ml·min-1。结果:线性范围是0．4-7．9μg(r=1．000 0),平均回收率为100．0％,RSD 0．1％。结论:此方法简便,快速,准确,可用于藿香正气水中橙皮苷的含量测定。
Objective: To establish a method for the determination of hesperidin in Huoxiangzhengqi water. Methods: The content of hesperidin in Huoxiangzhengqi was determined by HPLC. A Hypersil ODS C-18 reversed-phase column was used. The mobile phase was acetonitrile-8% acetic acid solution (10:90). The detection wavelength was 284 nm and the flow rate was 1.0 ml·min-1. Results: The linear range was 0.4-7.9 μg (r=1.000 0) with an average recovery of 100.0% and RSD 0.1%. Conclusion: This method is simple, rapid and accurate. It can be used for the determination of hesperidin in Huoxiangzhengqi water.