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针对光合细菌产氢过程中,菌种长期使用出现的菌种老化、产氢量下降、产氢不稳定等问题,采用菌体部分回流法提高光合细菌菌体活性,筛选出产氢活性较高的菌种用于产氢。试验结果表明,采用菌体部分回流法能有效提高光合细菌老化菌种的菌体活性,其最佳回流条件为:回流时间36h,回流量30%,回流周期为4次(192h)。通过回流活化,菌种的产氢能力明显增强,以牛粪污水为基质的产氢试验中,回流活化菌种产氢周期长,产氢稳定,168h累计产氢量达到282mL,比未回流菌种产氢量提高了80.7%,产气中平均氢含量达到58.6%,是未回流菌种产气中氢含量的1.21倍。
In the process of hydrogen production of photosynthetic bacteria, the strain of bacteria photosynthetic bacteria was enhanced by partial bacterial cell reflow to increase the activity of hydrogen-producing bacteria during the long-term use of bacteria strains aging, hydrogen production decreased, instability of hydrogen production, etc. Strain for hydrogen production. The results showed that the best reflux conditions were as follows: reflux time 36h, reflux 30%, reflux period 4 times (192h). The hydrogen production ability of the strain increased significantly by refluxing. In the hydrogen production test based on cow dung sewage, the hydrogen production cycle of the activated bacteria was long and the hydrogen production was stable. The cumulative hydrogen production reached 168 mL in 168 h, The hydrogen production increased by 80.7%, the average hydrogen content in the gas reached 58.6%, which was 1.21 times of the hydrogen content in the gas produced by the non-reflux species.