Dear Editor,Approximately every fifth Arabidopsis T-DNA insertion line contains chromosomal translocations caused by the inserted T-DNA (Clark and Krysan,2010).Albeit broad use of T-DNA lines for mutant analysis,little is known about the consequences of these chromosomal rearrangements and only a few studies describe chromosomal aberrations in the mutant lines.While plant growth in general is not affected by such chromosomal translocations,defects in gametophyte development have been observed in lines that are heterozygous for the T-DNA insertion (Ray et al.,1997;Curtis et al.,2009).These gametophytic defects are probably caused by interaction of two non-homologous chromosomes during meiosis resulting in the formation of tetravalents and subsequently unbalanced gametes after meiosis (Curtis et al.,2009).Importantly,the transmission of the T-DNA insertion through male and female gametophytes is not affected in the mutants,and plants that are homozygous for the translocation do not show aborted gametophytes anymore (Curtis et al.,2009).These results suggest that chromosomal translocations are easily overlooked when analyzing T-DNA lines.Here,we describe two previously uncharacterized T-DNA insertion alleles for the receptor-like kinase FERONIA (fer-6 and fer-7) and one T-DNA line for its pollen-specific homolog ANXUR2 (anx2-1),which comprise chromosomal translocations as assessed with genetic mapping experiments.These experiments also suggest a tentative mechanism for the deformed pollen phenotype.