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将(N-吡咯)己基硫醇自组装在金膜上,并采用化学氧化聚合法使吡咯与羧基功能化吡咯衍生物在自组装薄膜上进行共聚,然后对共聚物进行羧基活化处理.探针DNA通过与共聚物膜之间的共价键作用而固定在其表面上,接着与靶向DNA杂交.同时采用傅立叶红外变换光谱仪和X-射线光电子能谱仪对共聚物在DNA固定前后的化学组成进行详细表征.采用电化学工作站对DNA在共聚物薄膜上的固定/杂交前后所引起的电化学性质变化进行测试.还采用等离子表面谐振仪在位考察了DNA在共聚物膜上的固定动力学过程,进一步探明DNA的固定行为.结果表明DNA在导电吡咯共聚物薄膜上的固定是一缓慢过程,同时DNA固定/杂交也会引起共聚物薄膜的电化学活性和电荷转移能力的降低.
Self-assembly of (N-pyrrole) hexylmercaptan onto the gold film and the pyrrole and carboxyl functionalized pyrrole derivatives were copolymerized on the self-assembled film by chemical oxidative polymerization, and then the copolymer was activated by carboxyl. DNA was immobilized on its surface through covalent bond with the copolymer membrane and then hybridized with the target DNA.At the same time, the chemical changes of the copolymer before and after DNA immobilization were analyzed by using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy The composition was characterized in detail.Characteristics of the electrochemical properties caused by the immobilization / hybridization of DNA on the copolymer film before and after the electrochemical workstation were tested, and the immobilization of DNA on the copolymer film was also examined in situ using a plasma surface resonator The results showed that the immobilization of DNA on the conductive pyrrole copolymer film was a slow process, and DNA immobilization / hybridization also caused the decrease of electrochemical activity and charge transfer ability of the copolymer film.