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目的 研究新生大鼠缺氧缺血性脑损伤 (HIBD)发生后不同时段心肌细胞凋亡情况。方法 结扎新生 7日龄大鼠右颈总动脉后放入 8%低氧舱 2h制成HIBD模型 ,应用AnnexinV/PI双染流式细胞技术检测缺氧缺血 (HI)后 1、4、18、2 4、4 8、72h及 7d心肌细胞凋亡情况。结果 HI后各HI组心肌细胞的凋亡率均明显高于对照组 (P <0 .0 5 ) ,72hHI组凋亡率又显著高于其他组 (P <0 .0 5 ) ,表明HI后 7d内各组大鼠心肌均存在超出正常凋亡 ,尤以 72hHI组凋亡率最高。结论 新生大鼠HIBD后 1h～ 7d内心肌细胞均存在明显凋亡 ,以HI后 72h更明显 ,提示对缺氧缺血性脑病新生儿保护心肌减少心肌凋亡的治疗应至少用至生后 7d
Objective To investigate the apoptosis of myocardial cells in neonatal rats after hypoxic-ischemic brain damage (HIBD). Methods The right common carotid artery of neonatal 7-day-old rats was ligated and placed in 8% hypoxic chamber for 2 hours to make HIBD model. Annexin V / PI double staining flow cytometry was used to detect the hypoxic-ischemic (HI) , 2, 4, 8, 72h and 7d myocardial cell apoptosis. Results The apoptotic rate of HI in each HI group was significantly higher than that in control group (P <0.05), and the apoptosis rate in 72hHI group was significantly higher than that in other groups (P <0.05), indicating that after HI Within 7 days, all the myocardium in all groups had abnormal apoptosis, especially in 72hHI group. Conclusions The HIBD of newborn rats showed significant apoptosis of cardiomyocytes from 1h to 7d, which was more obvious at 72h after HI, suggesting that the treatment of hypoxic-ischemic encephalopathy neonatal myocardial protection to reduce myocardial apoptosis should be at least 7d after birth