[目的]探究新化合物HNPC-A14401的杀菌作用机理。[方法]通过研究HNPC-A14401活体杀菌活性,并通过扫描电镜观察其对菌丝影响,以及研究其对能量代谢影响,初步研究了HNPC-A14401的杀菌作用方式和机制。[结果]HNPC-A14401对黄瓜灰霉病活体防效与啶酰菌胺处同一水平,其对菌丝扩展有强烈抑制作用但对气生菌丝影响较小,其对能量代谢的影响不显著。[结论]HNPC-A14401作用方式可能为抑制病菌侵染或菌丝在细胞间扩展,其对病原菌能量代谢过程无影响。 [Objective] The research aimed to explore the bactericidal action mechanism of new compound HNPC-A14401. [Method] The bactericidal activity and the mechanism of HNPC-A14401 were studied through studying the bactericidal activity of HNPC-A14401 and observing its effect on mycelia by scanning electron microscopy and its effects on energy metabolism. [Result] The results showed that HNPC-A14401 had the same level of boscalid control efficacy against Botrytis cinerea, which had a strong inhibitory effect on the mycelium expansion but little effect on the aerial mycelium, which had no significant effect on energy metabolism . [Conclusion] The mode of action of HNPC-A14401 may be to inhibit the pathogen infection or the expansion of mycelium between cells, which has no effect on the energy metabolism process of pathogen.