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将28只家兔制成体温过低症动物模型,观察温水浴、电磁局部高温及自然复温的效果和试验中家兔血清CK(肌酸激酶)、LPO(脂质过氧化物)的变化。将动物Tr(直肠温)降至平均25.6℃,分别以40℃温水浴、13.56MHz的电磁能(约7W·kg~(-1))和自然复温法将Tr复温至35℃,温水浴法平均耗时75min,电磁法平均89min,自然复温法为470min。CK、LPO值在低体温期比试验前有所增加,复温后升高更加明显,且低体温时程越长,CK、LPO值升高越明显,CK值由试验前的123.99±34.68U/L到复温后的421.59±156.2U/L(P<0.01),LPO由试验前的MDA5.12±0.98nmol/ml上升到试验后11.04±1.05nmol/ml(P<0.01)。CK活力变化可推测组织细胞受损程度,复温后CK值升高说明低温对机体组织的继发效应。LPO值的变化提示低温复温时组织抗氧化能力减弱。
Twenty-eight rabbits were made animal models of hypohemia. The effects of warm water bath, local hyperthermia and natural rewarming were observed. Changes of rabbit serum CK (creatine kinase) and LPO (lipid peroxidation) . The animal Tr (rectal temperature) was reduced to an average of 25.6 ° C. The Tr was rewarmed to 35 ° C with warm water at 40 ° C, electromagnetic energy of 13.56 MHz (about 7 W · kg -1), and warm water The average bath time 75min, the average 89min electromagnetic method, the natural rewarming method 470min. The values of CK and LPO increased in hypothermia period before the experiment, and increased more obviously after rewarming. The longer the hypothermia duration was, the more obviously the CK and LPO increased. The value of CK increased from 123.99 ± 34.68U / L to 421.59 ± 156.2U / L after rewarming (P <0.01). LPO increased from 5.12 ± 0.98nmol / ml before test to 11.04 ± 1.05nmol / ml after test (P <0.01). Changes in CK activity can be speculated that the extent of tissue damage, after rewarming CK value shows the secondary effect of low temperature on the body tissue. The change of LPO value suggested that the anti-oxidation ability of the tissue was weakened when rewarming.