经眶后静脉窦途径移植小鼠骨髓细胞以检测多能造血干细胞(CFU-S)。应用此法检测CFU-S时,注入的骨髓有核细胞数与生成的脾结节数之间是良好的线性关系。与经典的尾静脉注入途径相比较,其优点是:容易掌握方法,操作时间缩短,成功率高,特别是用黑色或棕色小鼠作注射时,更能体现眶后静脉窦注射的这一优点。 Murine bone marrow cells were transplanted through the retro-orbital sinus to detect pluripotent hematopoietic stem cells (CFU-S). When this method was used to detect CFU-S, there was a good linear relationship between the number of nucleated bone marrow cells injected and the number of splenic nodules formed. Compared with the classic route of tail vein injection, the advantages are: easy to grasp the method, shorten the operation time, the success rate is high, especially when using black or brown mice for injection, better reflect this advantage of retro-orbital sinus injection .