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目的本研究旨在探讨长链非编码核糖核酸(lncRNA)与原发性醛固酮增多症发病机制间的相关性。方法选取2010-2016年间于我院诊断为醛固酮瘤(APA)的患者的组织标本为病例组(n=33),同时选取正常肾上腺皮质组织为对照组(n=19)。分别提取APA组织和正常肾上腺皮质组织各6例的RNA,利用lncRNA芯片技术检测两组lncRNA表达谱的差异,并对芯片结果进行统计学及生物信息学分析。采用编码-非编码(CNC)共表达网络获得差异表达的lncRNA,并在所有研究样本中进行实时聚合酶链反应验证。结果 lncRNA芯片共检测出差异表达的lncRNA 1 809条,其中1 181条表达上调,628条表达下调。功能分析提示,39条增强子样、69条基因间区和44条人类HOX基因位点的lncRNA存在表达差异。CNC共表达网络分析提示,6条与浦肯野细胞蛋白4表达相关的lncRNA中有5条存在表达差异(P<0.05),其中病例组与对照组uc003zpr.2表达差异最为明显。结论 APA组织与正常肾上腺皮质组织的lncRNA存在表达差异,uc003zpr.2在醛固酮调节中可能具有重要作用。
Objective This study aimed to investigate the relationship between long chain non-coding ribonucleic acid (lncRNA) and the pathogenesis of primary aldosteronism. Methods Tissue samples of patients with aldosteronoma (APA) diagnosed in our hospital from 2010 to 2016 were selected as case group (n = 33) and normal adrenal cortex tissue as control group (n = 19). The RNA of 6 cases of APA and normal adrenal cortex were extracted respectively. The difference of lncRNA expression between the two groups was detected by lncRNA microarray. The results of the chip were analyzed by statistics and bioinformatics. Differentially expressed lncRNAs were obtained using a coding-noncoding (CNC) co-expression network and verified by real-time polymerase chain reaction in all study samples. Results 1 809 differentially expressed lncRNAs were detected in lncRNAs, of which 1 181 were up-regulated and 628 down-regulated. Functional analysis suggested that there were differences in expression of lncRNA among 39 enhancer-like, 69 intergenic and 44 human HOX loci. The CNC co-expression network analysis indicated that there were 5 of the 6 lncRNAs related to Purkinje cell protein 4 expression (P <0.05), of which the difference between uc003zpr.2 and control group was the most significant. Conclusion The expression of lncRNA in APA tissue and normal adrenal cortex tissue is different, uc003zpr.2 may play an important role in the regulation of aldosterone.