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目的 研究透明质酸钠 (SH)在角膜保存中的作用 ,为眼库角膜保存方法的研究提供新思路。方法 分别在短期和中期保存中应用SH保存角膜 ,对保存不同时间的角膜行大体形态 ,活体内皮镜 ,苔盼兰 -茜素红染色 ,SDH、G -6-P酶检测和电镜观察。结果 短期和中期保存中实验组各项指标检测结果均明显好于对照组 ,差异有显著性 (P <0 0 1)。结论 SH对角膜内皮细胞有明显的保护和稳定作用 ,可有效用于角膜短期和中期保存 ,故有其重要的研究价值和应用前景。
Objective To study the role of sodium hyaluronate (SH) in preservation of cornea and provide new ideas for the study of cornea preservation in the eye bank. Methods SH preserved corneas were used in the short - term and medium - term preservation, respectively. The general morphology, in vivo endoscopy, hopepan - red alizarin red staining, SDH, G - 6 - P enzyme activity and electron microscopy were observed. Results In the short and medium term, the test results of each index in the experimental group were significantly better than those in the control group, with significant difference (P <0.01). Conclusion SH has obvious protective and stabilizing effects on corneal endothelial cells, which can be effectively used for short-term and mid-term preservation of corneas. Therefore, SH has its important research value and application prospect.