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目的:对申克孢子丝菌几丁质合成酶基因(chs1)及其编码蛋白的结构和特性进行分析和预测。方法:利用NCBI网站、Ex PASy和CBS等工具预测申克孢子丝菌chs1基因及编码蛋白的结构和功能。结果:chs1 mRNA全长2 745 bp,编码914个氨基酸。BLAST分析显示,申克孢子丝菌与巴西孢子丝菌、蓝菌属真菌、足马杜拉分枝菌等真菌chs氨基酸序列一致性达到99%,且有保守域。预测chs1蛋白相对分子量为102 714.7,理论等电点为6.71。预测chs1编码蛋白ɑ螺旋、β折叠、β转角、无规则卷的比例分别是38.40%、17.94%、10.39%、33.26%。chs1蛋白是一种疏水蛋白,包含9个跨膜区,未发现信号肽。结论:成功预测了chs1基因及编码蛋白质的生化性质及结构特征,为下一步对其进行克隆、表达和敲除奠定基础。
OBJECTIVE: To analyze and predict the structure and characteristics of the chs1 gene and its encoded protein of Sporotrichus. Methods: The structure and function of chs1 gene of Spirulina conidia and its encoded protein were predicted by NCBI website, Ex PASy and CBS. Results: The chs1 mRNA was 2 745 bp in length and encoded 914 amino acids. BLAST analysis showed that the conidia of S. schenckii shared 99% identity with the fungal chs from Sporotrichum sp., Pseudomonas spp. The relative molecular weight of chs1 protein was predicted to be 102 714.7 with a theoretical isoelectric point of 6.71. The predicted chs1 encoded proteins ɑ helix, β-sheet, β-angle, random coil ratio were 38.40%, 17.94%, 10.39%, 33.26%. The chs1 protein is a hydrophobin that contains nine transmembrane domains and no signal peptide is found. Conclusion: The biochemical properties and structural characteristics of chs1 gene and its encoded protein were successfully predicted, which laid the foundation for the cloning, expression and knockout of chs1 gene.