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本文采用聚丙烯酰胺凝胶电泳和光密度扫描法测定海拔7m,2300m,4300m健康人红细胞乳酸脱氢酶同工酶活性,酶谱的分离。结果与急性缺氧,低氧适应条件下人血清乳酸脱氢酶同工酶酶谱比较,红细胞呈现了特异酶谱。在扫描积分中,苏州组与西宁组的亚基百分活性及总酶活性相似,而苏州西宁两组与天峻木里组比较亚基相对百分活性及总酶活性有明显差异(P<0.05)。研究结果提示,海拔3000m以下人红细胞乳酸脱氢酶同工酶改变不大,随着海拔的增高,同工酶M型亚基活性增高,酶系统发生相应的代谢调节变化。乳酸脱氢酶同工酶自身调节可能对红细胞的高原缺氧适应起了较为重要作用。
In this paper, polyacrylamide gel electrophoresis and optical density scanning method for the determination of lactate dehydrogenase isoenzyme activity, enzyme zymogram separation of healthy red blood cells at 7m, 2300m, 4300m altitude. Results Compared with the enzyme profiles of human serum lactate dehydrogenase isozymes under acute hypoxia and hypoxia adaptation, the erythrocytes showed a specific enzyme pattern. In the scan scores, subunit activity and total enzyme activity in Suzhou group and Xining group were similar, while the relative percentage activity and total enzyme activity of subunits in Suzhou Xining and Tianjunmuli groups were significantly different (P < 0.05). The results suggest that human erythrocyte lactate dehydrogenase isoenzyme has little change under the altitude of 3000m, with the increase of altitude, the activity of isozymes M-type subunit is increased, and the corresponding metabolic regulation of enzyme system occurs. Lactate dehydrogenase isoenzyme itself may play an important role in the adaptation of erythrocytes to plateau hypoxia.