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目的探讨心脏舒张功能障碍中血清Ⅰ型前胶原羧基端肽(PⅠCP)和Ⅲ型前胶原氨基端肽(PⅢNP)水平的变化及其与心脏舒张功能障碍、胶原重构的关系。方法 60只兔随机均分为对照组、心肌梗死(MI)组、左室肥厚(LVH)组(n=20)。第2、4、8、12周ELISA检测PⅠCP、PⅢNP水平及多普勒血流和组织超声检测各组E/e’比值;第12周苦味酸天狼猩红染色偏振光法测定各组标本胶原容积分数(CVF)和Ⅰ/Ⅲ型胶原比值。结果与对照组比较,MI组和LVH组PⅠCP、PⅢNP水平明显升高(P<0.05),MI组与LVH组比较PⅠCP、PⅢNP水平亦明显升高(P<0.05),并随时间增加;与对照组比较,MI组和LVH组E/e’比值明显升高(P<0.05),并随时间增加;与对照组比较,MI组和LVH组CVF明显升高(P<0.05)。PⅠCP、PⅢNP水平与E/e’比值呈正相关(P<0.05)。PⅠCP/PⅢNP比值与Ⅰ/Ⅲ胶原比值呈正相关(P<0.05)。结论 PⅠCP、PⅢNP水平可以评估不同病理状态下心脏舒张功能的改变及心肌胶原重构情况。
Objective To investigate the changes of serum type Ⅰ procollagen carboxy terminal telopeptide (PⅠCP) and type Ⅲ procollagen aminoterminal peptide (PⅢNP) in diastolic dysfunction and its relationship with diastolic dysfunction and collagen remodeling. Methods Sixty rabbits were randomly divided into control group, myocardial infarction (MI) group and left ventricular hypertrophy (LVH) group (n = 20). The levels of PⅠCP and PⅢNP in the 2nd, 4th, 8th and 12th weeks were detected by ELISA, and the E / e ’ratio of each group was detected by Doppler flow and tissue ultrasonography. At the 12th week, picric acid Volume fraction (CVF) and type Ⅰ / Ⅲ collagen ratio. Results Compared with the control group, the levels of PⅠCP and PⅢNP in MI group and LVH group were significantly increased (P <0.05), and the levels of PⅠCP and PⅢNP in MI group and LVH group were also significantly increased (P <0.05) In the control group, the ratio of E / e ’in MI group and LVH group was significantly increased (P <0.05) and increased with time; CVF in MI group and LVH group was significantly higher than that in control group (P <0.05). PⅠCP, PⅢNP levels and E / e ’ratio was positively correlated (P <0.05). PⅠCP / PⅢNP ratio was positively correlated with Ⅰ / Ⅲ collagen ratio (P <0.05). Conclusions The levels of PⅠCP and PⅢNP can assess the changes of cardiac diastolic function and myocardial collagen remodeling under different pathological conditions.