实验采用了50个经过长期大面积种植的棉花种质材料,利用改良的种子贮藏蛋白电泳技术进行棉花品种的鉴定研究。改良后的种子蛋白提取方案,克服了棉花种子中含油脂较多影响电泳结果的问题;采用连续体系的阴极电泳,使电泳周期缩短为2.5h,简化了操作步骤,重复性好,谱带清晰,有较高的多样性。 Fifty cotton germplasm materials, which have been planted over a long period of time, were used in the experiment. The improved seed storage protein electrophoresis technique was used to identify cotton varieties. The improved seed protein extraction scheme overcomes the problem that the oil content in the cotton seeds greatly affects the electrophoresis result; the cathodic electrophoresis in the continuous system shortens the electrophoresis cycle to 2.5 hours, simplifies the operation steps, has good repeatability and clear band , There is a high degree of diversity.