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以马氏珠母贝选系F2为实验材料,按照马氏珠母贝常规技术进行插核手术,并经过池塘休养和海区养殖过程,比较了细胞小片黏液处理和保养对马氏珠母贝育珠效果影响。实验设为4组:细胞小片黏液处理组(A)、细胞小片保养组(B)、同时进行细胞小片黏液处理和保养组(C),对照组(D),同时利用H E染色观察比较A和D组细胞小片显微结构的变化。研究结果表明,对照组细胞小片的外套膜边缘上皮细胞部分脱落,而处理组的外套膜边缘上皮细胞保持完整。育珠期结束后,A、B、C和D组育珠贝的成活率没有显著差别(P>0.05);A、B、C和D组留核率、商品珠率和优质珍珠率存在显著性差异(P<0.05),其中B组具有最大的商品珠率(81.4%)和优质珠率(34.6%),C组具有最小的商品珠率(70.7%)和优质珠率(20.6%)。本研究结果说明经过细胞小片保养处理能显著提高马氏珠母贝的商品珠率和优质珠率,这为优质海水珍珠生产提供了依据。
The selection of F2 was used as experimental material, and the insertion of F2 was carried out according to the conventional technique of M. jehoeba. After pond rearing and sea area breeding, Effect of beads effect. The experimental group was divided into 4 groups: the mucosa-treated group (A), the preserved group (B), the mucosa-treated and maintained group (C) and the control group (D) Changes in the microstructure of D group. The results showed that the epithelial cells of the mantle edge of the control group were partially shed, while the epithelium of the mantle edge of the treatment group remained intact. There was no significant difference in the survival rates of A, B, C and D (P> 0.05) at the end of the incubation period, while there were significant differences in the rate of nuclear retention, the commodity rate and the quality of pearl in groups A, B, C and D (P <0.05). Group B had the largest commercial bead rate (81.4%) and high quality bead rate (34.6%), the lowest commercial bead rate (70.7%) and high quality bead rate (20.6% . The results of this study indicate that the treatment of the cell pellet can significantly improve the product bead yield and the excellent bead rate of the mother pearl, which provides the basis for the production of the fine seawater pearl.