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目的了解山东省不同地理种群淡色库蚊对拟除虫菊酯类杀虫剂的抗药性水平和kdr等位基因的频率分布,探讨不同地理种群淡色库蚊kdr等位基因频率同抗药性水平之间的相关性。方法采用WHO推荐浸渍法检测蚊虫抗药性,采用AS·PCR扩增技术检测成蚊的kdr等位基因频率,利用线性回归方法分析kdr等位基因频率与抗药性的相关性。结果济南、淄博、济宁3个地理种群淡色库蚊对溴氰菊酯均产生抗药性,抗性系数分别是4.14、5.29和2.86;淄博种群对高效氯氰菊酯产生抗药性,抗性系数为48.68,济南种群对氯氰菊酯产生抗药性,抗性系数为4.36。济南、淄博、烟台、济宁种群淡色库蚊的kdr频率(%)分别是是44.71%、54.22%、21.84%、32.97%,淄博种群最高,其次是济南种群。不同种群的溴氰菊酯抗性水平与抗性等位基因频率有显著的相关性(r2=0.981 6,P=0.009 2),高效氯氰菊酯和氯菊酯抗性水平与抗性等位基因频率无显著的相关性(r2分别为0.590 4和0.020 1,P值分别为0.231 6和0.858 2)。结论山东省4个不同地理种群淡色库蚊对拟除虫菊酯类药物的抗性水平由高到底依次为淄博种群、济南种群、济宁种群、烟台种群,对3种药物都有不同程度的抗药性产生,其中溴氰菊酯抗性水平最高,其次是高效氯氰菊酯和氯氰菊酯;野外种群淡色库蚊对溴氰菊酯抗性水平与kdr等位基因频率呈显著相关性,高效氯氰菊酯、氯氰菊酯抗性水平与kdr等位基因频率无显著相关性。
Objective To understand the resistance level of pyrethrum insecticides and the frequency distribution of kdr alleles in Culex pipiens pallens from different geographical populations in Shandong Province and to explore the relationship between the frequency of kdr allele and the resistance level of Culex pipiens pallens in different geographical populations Correlation. Methods The resistance of mosquitoes was detected by the WHO recommended impregnation method. The kdr allele frequency of adult mosquitoes was detected by AS · PCR amplification. The correlation between kdr allele frequency and drug resistance was analyzed by linear regression. Results The resistance of deltamethrin to Culex pipiens pallens in three geographical populations of Jinan, Zibo and Jining was 4.14, 5.29 and 2.86, respectively. The population of Zibo was resistant to beta-cypermethrin and the resistance coefficient was 48.68. Jinan The population was resistant to cypermethrin with a resistance coefficient of 4.36. The kdr frequencies (%) of Culex pipiens pallens in Jinan, Zibo, Yantai and Jining were 44.71%, 54.22%, 21.84% and 32.97% respectively, with the highest in Zibo and the next in Jinan. There was a significant correlation between deltamethrin resistance and resistance allele frequency in different populations (r2 = 0.981 6, P = 0.009 2). The relative levels of beta-cypermethrin and permethrin resistance and allele frequency There was no significant correlation (r2 0.5904 and 0.0201 respectively, P values 0.231 6 and 0.858 2, respectively). Conclusion The resistance level of pyrethrum to Culex pipiens pallens from four different geographical populations in Shandong province were Zibo population, Jinan population, Jining population and Yantai population in the high to low order, with different degrees of resistance to three drugs , Of which deltamethrin was the highest, followed by beta-cypermethrin and cypermethrin. There was a significant correlation between deltamethrin resistance and kdr allele frequency in field populations of Culex pipiens pallens, and beta-cypermethrin and cypermethrin resistance There was no significant correlation between kdr allele frequencies.