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不同碘营养状态Wistar大鼠分别于饲养3、6、12个月时处死,放射免疫法测定血清甲状腺激素水平。放射性核素分析法检测大鼠脑组织Ⅱ型脱碘酶(DⅡ)和肝组织Ⅰ型脱碘酶(DⅠ)的活性。结果显示在早期碘缺乏状态下,动物出现以低T_4为主要表现的甲减,DⅠ和DⅡ活性代偿性上调。高碘在转录后直接抑制DⅠ的活性,使血清TT_3和FT_3下降,而DⅡ的活性增高,可能是由于T_4和T_3对酶的底物诱导失活所致。
Wistar rats with different iodine nutrition status were sacrificed at 3, 6 and 12 months respectively, and serum thyroid hormone levels were measured by radioimmunoassay. The activity of type Ⅱ deiodinase (D Ⅱ) and type Ⅰ deiodinase (D Ⅰ) in rat brain were detected by radionuclide analysis. The results showed that hypothyroidism with hypothyroidism, hypothyroidism, D Ⅰ and DⅡ activity compensatory upregulation were observed in animals with early iodine deficiency. High iodine directly inhibits the activity of DⅠ after transcribing, the serum TT_3 and FT_3 decreased, while the activity of DⅡ increased. This may be due to the inactivation of substrates by T_4 and T_3.