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为创建大白菜—结球甘蓝易位系,以大白菜—结球甘蓝9号单体异附加系(AC9)为试材,对其与大白菜亲本‘85-1’回交后代植株进行游离小孢子培养。利用结球甘蓝9号染色体相对应的C06连锁群特异的20个InDel标记对小孢子植株进行筛选,结合细胞学鉴定从中获得两个添加甘蓝9号染色体不同片段的易位系植株‘AT9-1’和‘AT9-2’。通过对InDel标记的加密设计,明确了易位系‘AT9-2’中甘蓝片段大小为1.03 Mb;利用大白菜A基因组10个连锁群上均匀分布的177个InDel标记对其进行分子鉴定,初步确定了易位系‘AT9-2’中甘蓝染色体片段位于大白菜1号染色体上。对易位系植株‘AT9-1’和‘AT9-2’分别进行自交、与大白菜亲本‘85-1’回交、与大白菜高代自交系‘14-28’和‘14-36’杂交,利用结球甘蓝C06连锁群特异的InDel标记对自交、回交、杂交后代进行鉴定,结果表明:两个易位系中甘蓝9号染色体片段的遗传稳定性非常低,易位系‘AT9-1’的自交、回交和杂交后代中含有甘蓝染色体片段的植株比例仅为8.9%、3.1%和2.8%;而‘AT9-2’仅为6.7%、1.6%和2.6%。
In order to create a Chinese cabbage-Brassica campestris translocation line, the Chinese cabbage-cabbage 9 accession line (AC9) Microspore culture. Microspore plants were screened by using 20 InDel markers specific to the C06 linkage group corresponding to chromosome 9 in cabbage and combined with cytological identification to obtain two transposable plant lines AT9-1 ’And’ AT9-2 ’. Through the encryption design of InDel marker, the size of the cabbage fragment in ’AT9-2’ was determined to be 1.03 Mb. 177 InDel markers evenly distributed on the 10 linkage groups of Chinese cabbage A genome were used for molecular identification It was confirmed that the chromosome fragment of cabbage in ’AT9-2’ of the translocation line was located on the chromosome 1 of Chinese cabbage. The translocation lines of ’AT9-1’ and ’AT9-2’ of the translocation lines were backcrossed with the parents ’85-1 of Chinese cabbage and crossed with the Chinese cabbage lines ’14 -28’ and ’14 - The results showed that the genetic stability of Brassica napus chromosome 9 in the two translocation lines was very low and the translocation The percentage of plants containing the cabbage chromosome fragment was only 8.9%, 3.1% and 2.8% in the self-cross, backcross and hybrid progenies of ’AT9-1’, while only 6.7%, 1.6% and 2.6% .