大豆籽粒色氨酸測定(P—DMAB)法的研究

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目前,色氨酸国内外广泛应用的方法有离子交换色层法、二羟基乙酸法(乙醛酸法)、对二甲胺基苯甲醛(P-DMAB)酸解及碱解分光光度法、萤光法等。Milker(1967)将P-DMAB程序用于谷物饲料的常规分析,得到谷物中色氨酸回收牢为80%, At present, tryptophan methods widely used at home and abroad are ion exchange chromatography, glyoxylic acid method (glyoxylic acid method), p-DMAB acid hydrolysis and alkali hydrolysis spectrophotometry, Fluorescent method and so on. Milker (1967) used the P-DMAB program for routine analysis of cereal feedstuffs, resulting in an 80% recovery of tryptophan in cereals,
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