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目的:探讨“抗肝纤1号”的抗肝纤维化作用。方法:昆明种小白鼠170只,分成正常(N)对照组、CCl_4中毒(1)对照组,CCl_4中毒同时给桃仁(A),秋水仙碱(C)治疗对照组,“抗肝纤1号”方小剂量(H_1)、大剂量(H_2)治疗组。分期分批剖杀。用排液法测量脾脏体积,取肝脏作肝组织切片,HE染色光镜检查;图象分析法测肝脏切片中胶原纤维相对比面积;生化法测肝组织脯氨酸羟化酶(LRHL)活性、脯氨酸(Pro)、羟脯氨酸(Pyp)的含量。结果:N组肝组织属正常结构。Ⅰ组肝脏细胞变性、坏死及纤维组织增生均比N组及其他治疗组重(P<0.05～0.01),脾脏(0.187±0.034ml)比治疗组均大(P>0.05),Ⅰ组动物肝脏胶原纤维相对面积(2.75±0.38％)最大,与其他各组比均有显著差异(P<0.05～0.01),H_2组最小(0.90±0.29％),与N组相近(P>0.05)。LPHL活性亦以Ⅰ组最高(2.738±0.661U/g肝可溶性蛋白),H组均较低,尤以H_2组(1.38±0.22U/g肝可溶性蛋白)最低,与N组在早期相近(P>0.05)。肝组织的Pro、Pyp在总氨基酸(TAA)中的含量亦Ⅰ组为最高(1.47±0.26％,P<0.01),H_2组最低(0.48±11％,P<0.05～0.01)。结论:①桃仁、秋水仙碱、抗肝纤1号方均有抗肝纤维化的作用,但抗肝纤1号方的疗效比前两者好;②在抗肝纤1号方中又以H_2组为最佳,提示活血化瘀、改善肝微循环?
Objective: To investigate the anti-fibrotic effect of “anti-liver fiber 1”. METHODS: 170 Kunming mice were divided into normal (N) control group and CCl_4 poisoning (1) control group. CCl_4 poisoning was also given to peach kernel (A) and colchicine (C) to the control group. "Square small dose (H_1), high dose (H_2) treatment group. Phased killing in batches. The volume of spleen was measured by drainage method. The liver was taken for liver biopsy. HE staining was used for light microscopy; image analysis method was used to measure the relative area of collagen fibers in liver slices; biochemical method was used to measure liver valine hydroxylase (LRHL) activity. , Proline (Pro), Hydroxyproline (Pyp) content. Results: The liver tissue of group N was normal structure. The liver cell degeneration, necrosis and fibrous tissue hyperplasia in group I were heavier than those in group N and other treatment groups (P<0.05-0.01), and spleen (0.187±0.034 ml) was greater than that in treatment group (P>0.05). The relative area of collagen fibers (2.75±0.38%) was the largest, which was significantly different from that of other groups (P<0.05-0.01). The H2 group was the smallest (0.90±0.29%) and similar to N group (P>0.05). LPHL activity was also highest in group I (2.738±0.661 U/g hepatic soluble protein), and was lower in group H, especially in group H2 (1.38±0.22 U/g hepatic soluble protein), which was similar to group N in the early stage (P >0.05). The contents of Pro and Pyp in total liver amino acid (TAA) were also highest in group I (1.47±0.26%, P<0.01), and lowest in H_2 group (0.48±11%, P<0.05-0.01). Conclusion: 1 Taoren, colchicine, and anti-liver fiber No.1 have anti-fibrosis effect, but anti-liver fiber No.1 is more effective than the former two; 2 is also used in anti-liver fiber No.1. H2 group is the best, suggesting that blood circulation, improve liver microcirculation?