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目的:探讨胞壁酰二肽(MDP)激活大鼠巨噬细胞抗肿瘤效应的途径和机制。方法:以SD大鼠为骨肉瘤动物模型进行肿瘤抑制试验;采用中性红吞饮实验、间接MTT法及硝酸还原酶法,测定大鼠腹腔巨噬细胞的功能。结果:MDP(50μg～100μg/鼠)皮下注射可明显抑制UMR106骨肉瘤细胞在SD大鼠体内的生长,并可显著提高巨噬细胞的吞噬功能、杀伤活性及TNF和NO的分泌水平,且呈现一定的量效关系。结论:MDP激活的巨噬细胞可能是机体非特异性免疫抗瘤效应的主要基础。
Objective: To explore the pathway and mechanism of anti-tumor effect of murine macrophage activated by muramyl dipeptide (MDP). Methods: The SD rats were used as the animal model of osteosarcoma to carry out the tumor inhibition test. The function of rat peritoneal macrophages was determined by the neutral red swallowing experiment, the indirect MTT assay and the nitrate reductase method. RESULTS: Subcutaneous injection of MDP (50 μg to 100 μg/mouse) significantly inhibited the growth of UMR106 osteosarcoma cells in SD rats, and significantly increased the phagocytosis, killing activity and TNF and NO secretion of macrophages. A certain dose-effect relationship. Conclusion: MDP-activated macrophages may be the main basis for nonspecific immune and anti-tumor effects in the body.