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为了探讨一氧化氮 (NO)在结肠癌发生发展中的作用。方法 :采用地高辛标记的诱导型一氧化氮合成酶(iNOS)mRNA探针 ,对 2 0例经结肠镜活检、手术及病理证实的结肠腺癌进行原位杂交 ,以显示细胞内iN OSmRNA的表达情况 ,并与结肠息肉 ,结肠腺瘤进行比较。结果 :2 0例结肠腺癌患者中表达iNOSmRNA杂交信号强阳性 (+ + + ) 9例 ,中度阳性 (+ + ) 4例 ,弱阳性 (+ ) 4例。阴性 (- ) 3例 ,总阳性率达 85 % ,而结肠息肉和结肠腺瘤则多为阴性或弱阳性 ,两者比较有显著性差异 (P <0 .0 0 5 )。结论 :实验结果表明大多数结肠癌患者的癌细胞表达iNOSmRNA。提示结肠癌细胞自身能产生一氧化氮 ,而内源性NO产量的多少 ,在结肠癌的发生、发展及转归过程中可能具有密切关系
In order to investigate the role of nitric oxide (NO) in the development of colon cancer. Methods: Inducible nitric oxide synthase (iNOS) mRNA probes labeled with digoxigenin were used to in situ hybridize 20 colon adenocarcinomas confirmed by colon biopsy, surgery and pathology to show the expression of intracellular iNOS mRNA Of the expression, and with colon polyps, colon adenomas were compared. Results: There were 9 (+ +) positive iNOS mRNA positive signals in 4 of 20 cases of adenocarcinoma of colon adenocarcinoma, 4 cases of moderate positive (+) and 4 cases of weak positive (+). Negative (-) in 3 cases, the total positive rate of 85%, while colon polyps and colon adenomas are mostly negative or weak positive, the two were significantly different (P <0.05). CONCLUSIONS: The experimental results indicate that most colon cancer patients express iNOS mRNA. Suggesting that colon cancer cells can produce nitric oxide by themselves, while the amount of endogenous NO production may play an important role in the occurrence, development and prognosis of colon cancer