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目的:建立血瘀型非酒精性脂肪性肝病(NAFLD)病证结合模型。方法:70只大鼠随机分正常组10只、NAFLD组30只及血瘀型NAFLD组30只,正常组以大鼠标准饲料喂养,其余两组以高脂饲料喂养,同时,血瘀型NAFLD组联合应用去甲肾上腺素(NE)加小牛血清白蛋白(BSA)进行复合造模,在4、8、12周末3个时相点处理大鼠。观察大鼠血瘀体征,DRT4激光多普勒血流测定仪检测皮肤微循环,全自动血液流变快测仪检测血液流变学,全自动生化仪检测血脂及血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平,HE染色观察肝脏脂肪变和炎症程度。结果:血瘀型NAFLD大鼠出现舌下静脉曲张、舌质青紫有瘀斑、眼球暗红、尾部瘀斑等血瘀证候以及皮肤微循环障碍、血液流变学异常、血脂紊乱、肝组织脂肪变和炎细胞浸润等;血瘀型NAFLD大鼠血瘀体征积分及全血黏度、全血还原黏度及红细胞聚集指数较NAFLD组明显增高。结论:高脂饮食、NE及BSA复合因素可成功建立血瘀型NAFLD模型,该模型既具备非酒精性脂肪性肝病的病理特征,又符合中医血瘀证的证候表现。
Objective: To establish a blood-stasis non-alcoholic fatty liver disease (NAFLD) disease syndrome model. Methods: Seventy rats were randomly divided into normal group (n = 10), NAFLD group (n = 30) and blood stasis type NAFLD group (n = 30). Normal rats were fed with standard diet and the remaining two groups were fed with high fat diet. The rats were treated with NE and bovine serum albumin (BSA) in combination mode. The rats were treated at 3, 4, 8 and 12 weeks. Blood stasis signs were observed in rats, skin microcirculation was detected by DRT4 laser Doppler flowmetry, hemorheology was detected by automatic hemorheology detector, serum lipid and serum alanine aminotransferase ALT), aspartate aminotransferase (AST) levels were observed by HE staining to observe the degree of hepatic steatosis and inflammation. Results: Blood stasis type NAFLD rats developed sublingual varicose veins, ecchymosis of the tongue, dark redness of the eyes and ecchymosis of the tail, as well as microcirculation disorders, abnormal blood rheology, dyslipidemia, liver tissue Steatosis and inflammatory cell infiltration. Blood stasis syndrome, blood viscosity, whole blood viscosity and erythrocyte aggregation index of blood stasis type NAFLD rats were significantly higher than NAFLD group. CONCLUSION: The combination of high-fat diet, NE and BSA can successfully establish the NAFLD model of blood stasis. This model not only has the pathological features of non-alcoholic fatty liver disease but also complies with the TCM syndromes of blood stasis syndrome.