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目的 探讨慢乙肝患者血清HBVDNA的表达与肝细胞内HBsAg和HBcAg表达的相关性。方法 应用定量PCR技术检测 170例乙肝患者的血清HBVDNA ,同时应用免疫组化技术测定其肝细胞内HBsAg及HBcAg含量。结果 ⑴慢乙肝患者不论血清HBVDNA是否阳性 ,肝细胞内均可检出HBsAg ;但HBVDNA载量低者 ,肝细胞内HBsAg含量较低 (P <0 0 5 )。血清HBVDNA阴性者 ,HBcAg无一例检出。⑵血清HBVDNA载量越高 ,患者肝细胞内HBcAg的阳性率明显升高 (P <0 0 5 )。⑶肝组织炎症G2期以上的慢乙肝患者血清HBVDNA阳性较阴性的多 (P <0 0 5 ) ,但并不与HBVDNA载量高低相伴随 (P >0 0 5 )。而G2期以上的慢乙肝患者肝细胞内HBcAg的阳性率明显升高 (P <0 0 5 )。结论 慢乙肝患者血清HBVDNA的表达与肝细胞内HBsAg和HBcAg表达密切相关。肝细胞内HBsAg的表达是筛查HBV感染的敏感指标 ,HBcAg是评价HBVDNA复制程度的较可靠指标。
Objective To investigate the relationship between the expression of HBVDNA and the expression of HBsAg and HBcAg in hepatocytes in patients with chronic hepatitis B (HBV). Methods Serum HBVDNA was detected by quantitative PCR in 170 patients with hepatitis B, and the content of HBsAg and HBcAg in hepatocytes was detected by immunohistochemistry. Results (1) HBsAg was detected in hepatocytes in patients with chronic hepatitis B, regardless of whether HBVDNA was positive or not. However, the HBsAg content in hepatocytes was lower in those with lower HBV DNA load (P <0.05). Serum HBVDNA negative, no HBcAg detected. (2) The higher the serum HBV DNA load, the higher the positive rate of HBcAg in hepatocytes (P <0.05). (3) Serum HBVDNA in patients with chronic hepatitis B over the G2 phase of liver tissue inflammation is more negative (P <0.05), but not with the level of HBVDNA (P> 0.05). The positive rate of HBcAg in hepatocytes of patients with chronic hepatitis B over G2 was significantly higher (P <0.05). Conclusion The expression of HBVDNA in patients with chronic hepatitis B is closely related to the expression of HBsAg and HBcAg in hepatocytes. HBsAg expression in hepatocytes is a sensitive indicator of screening for HBV infection, HBcAg is a more reliable indicator of the degree of HBVDNA replication.