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目的建立一种基于自由基反应荧光增强的葡萄糖检测方法。方法葡萄糖分子被葡萄糖氧化酶氧化,酶反应产物H_2O_2和Fe~(3+)一起组成Fenton试剂,Fe~(3+)催化H_2O_2产生羟基自由基,催化产物迅速的和OPDA反应生成一种荧光低聚物并产生增强的荧光信号,实现葡萄糖的间接检测。结果在0.20~12.50 mmol/L浓度范围内,荧光强度和葡萄糖浓度呈线性关系,相关系数r=0.98,检测限为25μmol/L。结论该方法具有较高的灵敏度和简便的操作步骤,并且易于实现定量分析。
Objective To establish a glucose detection method based on free radical reaction fluorescence enhancement. Methods The glucose molecules were oxidized by glucose oxidase. The enzyme reaction products H 2 O 2 and Fe 3+ formed Fenton reagent. Fe 3+ catalyzed the production of hydroxyl radicals by H 2 O 2. The catalytic products rapidly reacted with OPDA to produce a low fluorescence Polymer and produce an enhanced fluorescence signal for indirect detection of glucose. The results showed that there was a linear relationship between fluorescence intensity and glucose concentration in the range of 0.20-12.50 mmol / L with a correlation coefficient of 0.98 and a detection limit of 25 μmol / L. Conclusion The method has high sensitivity and easy operation steps, and easy to achieve quantitative analysis.