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目的:探讨抑癌基因PTEN和癌基因c-erbB-2蛋白在子宫内膜癌组织中表达及其与临床病理特征的关系。方法:采用免疫组化法检测52例子宫内膜癌组织、29例子宫内膜不典型增生和18例正常子宫内膜组织中c-erbB-2及PTEN蛋白的表达,并分析其与临床特征的相关性。结果:子宫内膜癌组织中PTEN表达缺失率明显高于子宫内膜不典型增生和正常子宫内膜,P<0.001;PTEN表达缺失与不典型增生的严重程度、病理分级相关(P<0.05),但与肌层浸润深度和淋巴结转移无关(P>0.05)。子宫内膜癌组织中c-erbB-2的阳性表达率明显高于正常子宫内膜和子宫内膜不典型增生,P<0.05;c-erbB-2阳性表达与子宫内膜癌的病理分级、临床分期、肌层浸润深度和淋巴转移相关(P<0.05),但与不典型增生的程度无关(P>0.05)。在子宫内膜癌组织中PTEN的表达缺失率与c-erbB-2的过度表达呈正相关。结论:PTEN蛋白的表达缺失与子宫内膜癌的发生有关;c-erbB-2蛋白的高表达提示预后不良;PTEN和c-erbB-2联合检测可作为筛选复发转移高危因素的标志。
Objective: To investigate the expression of tumor suppressor gene PTEN and oncogene c-erbB-2 in endometrial carcinoma and its relationship with clinicopathological features. Methods: The expressions of c-erbB-2 and PTEN protein in 52 cases of endometrial carcinoma, 29 cases of endometrial dysplasia and 18 cases of normal endometrium were detected by immunohistochemistry and analyzed with clinical features Relevance. Results: The loss of PTEN in endometrial carcinoma was significantly higher than that in endometrial dysplasia and normal endometrium (P <0.001). The loss of PTEN expression was associated with the severity of atypical hyperplasia and pathological grade (P <0.05) , But not with the depth of myometrial invasion and lymph node metastasis (P> 0.05). The positive rate of c-erbB-2 in endometrial carcinoma was significantly higher than that in normal endometrium and endometrial dysplasia (P <0.05). The positive expression of c-erbB-2 and the pathological grade of endometrial carcinoma, Clinical stage, depth of myometrial invasion and lymphatic metastasis (P <0.05), but not with the degree of dysplasia (P> 0.05). In endometrial carcinoma, the loss of PTEN expression was positively correlated with the overexpression of c-erbB-2. CONCLUSION: The loss of PTEN expression is associated with the occurrence of endometrial carcinoma. The high expression of c-erbB-2 protein suggests a poor prognosis. The combined detection of PTEN and c-erbB-2 may be used as a marker to screen the risk factors for recurrence and metastasis.