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目的 :探讨尿标本中视黄醇结合蛋白(RBP)、转铁蛋白(TRF)、微量白蛋白(mALB)及Ⅳ型胶原(Ⅳ C)检测在糖尿病肾病早期诊断中的作用。方法 :用ELISA法检测尿中RBP、TRF及Ⅳ C ,免疫比浊法检测尿微量白蛋白(mALB) ,酶偶联法测定尿肌酐。结果 :正常对照组中尿RBP/Cr、TRF/Cr及Ⅳ C的含量分别为(2.12±0.95)mg/mmol,(1.81±0.52)mg/mmol,(44.68±17.2)ng/ml。在糖尿病无肾病组中 ,其微量白蛋白的含量与正常对照组相近(P>0.05) ,而RBP、TRF及Ⅳ C的含量与正常对照组相比具有显著差别(P<0.05)。在糖尿病的初期肾病组和临床肾病组 ,mALB、RBP/Cr、TRF/C的及Ⅳ C的含量均明显高于正常对照组(P<0.01)。结论 :联合检测尿RBP、TRF及Ⅳ C是诊断糖尿病肾病早期损伤灵敏、可靠的实验室指标。
Objective: To investigate the role of RBP, TRF, mALB and Ⅳ C in the early diagnosis of diabetic nephropathy. Methods: Urinary RBP, TRF and IVC were detected by ELISA, urine microalbumin (mALB) by immunoturbidimetric assay and urinary creatinine by enzyme coupling assay. Results: The urinary RBP / Cr, TRF / Cr and IV C contents in the normal control group were (2.12 ± 0.95) mg / mmol and (1.81 ± 0.52) mg / mmol and (44.68 ± 17.2) ng / ml, respectively. In diabetic nephropathy group, the content of microalbumin was similar to that in normal control group (P> 0.05), while the levels of RBP, TRF and IV C were significantly different from those in normal control group (P <0.05). The levels of mALB, RBP / Cr, TRF / C and IV C in the primary nephropathy group and the clinical nephropathy group were significantly higher than those in the normal control group (P <0.01). Conclusion: Combined detection of urinary RBP, TRF and Ⅳ C is a sensitive and reliable laboratory indicator for the early diagnosis of diabetic nephropathy.