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目的:口腔鳞癌是口腔颌面部常见的恶性肿瘤之一,本研究以侧群细胞为肿瘤干细胞突破口,通过检测、分选口腔鳞癌细胞系NTCR中侧群细胞(side population,SP)细胞亚群,深入研究不同细胞亚群的体内、外相关生物学特性,寻找口腔鳞癌中肿瘤干细胞存在的证据。方法:选取口腔鳞癌细胞系NTCR作为研究对象,Hoechst 33342染色后行流式细胞仪检测,分选口腔鳞状细胞癌中的SP细胞和非SP细胞,进行体外培养、长期分化和体内成瘤实验,对2种亚群细胞的体内和体外生物学特性进行检测和比较。结果:口腔鳞状细胞癌细胞系NTCR中含有9.3%SP细胞,其SP细胞在细胞的增殖能力、自我更新能力及裸鼠体内成瘤能力等方面与干细胞特性相似。结论:SP细胞可以认为是肿瘤干细胞的富集。进一步深入研究,有可能作为口腔鳞癌诊断、治疗和预后的靶标。
OBJECTIVE: Oral squamous cell carcinoma (OSCC) is one of the common malignant tumors in oral and maxillofacial region. In this study, side population cells were used as tumor stem cell breakthrough to detect and sort the side population (SP) cells of oral squamous cell carcinoma cell line NTCR Subpopulations, in-depth study of different cell subsets in vitro and in vivo related biological characteristics, looking for oral squamous cell carcinoma in the presence of evidence of stem cells. Methods: Oral squamous cell carcinoma cell line NTCR was selected as the research object. After being stained with Hoechst 33342, the flow cytometry was used to detect the SP cells and non-SP cells in oral squamous cell carcinoma, and the in vitro culture, long-term differentiation and in vivo tumorigenesis Experiments were performed to detect and compare the biological characteristics of two subpopulations in vitro and in vivo. Results: The oral squamous cell carcinoma cell line NTCR contained 9.3% of SP cells. The characteristics of the SP cells were similar to those of stem cells in cell proliferation, self-renewal and tumorigenicity in nude mice. Conclusion: SP cells can be considered as cancer stem cell enrichment. Further in-depth study, it may be as a diagnosis, treatment and prognosis of oral squamous cell carcinoma.