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目的明确mtDNA4977bp大片段缺失在肺癌组织、癌旁正常组织及非癌患者肺组织中的分布频率,探讨mtDNA4977bp大片段缺失与肿瘤的关系。方法采用长距离PCR方法对37例肺癌组织和相应的癌旁正常组织、20例非癌患者正常肺组织mtDNA4977bp缺失进行了检测。结果肺癌组织、癌旁正常肺组织及非癌患者正常肺组织中均检测出存在有线粒体DNA4977bp片段缺失。37例肺癌组织标本中有20例(54.1%)检测到4977bp缺失,37例相应的癌旁肺组织22例(59.5%)有缺失,其中8例在肺癌组织中未显示而却在癌旁正常组织检测到缺失,20例非癌患者正常肺组织中也有6例出现4977bp片段缺失。线粒体DNA4977bp缺失与年龄、吸烟因素有关。结论线粒体DNA4977bp缺失并非肺癌特异性突变,在癌变过程中不太可能起重要的作用,而可能仅仅是肿瘤发生发展过程中环境和遗传因素作用的反映。
Objective To determine the frequency of mtDNA 4977bp fragment deletion in the lung tissue, adjacent normal tissues, and non-cancerous lung tissues, and to explore the relationship between mtDNA 4977bp deletions and tumors. Methods Long-distance PCR was used to detect the loss of mtDNA4977bp in 37 cases of lung cancer tissues, corresponding adjacent normal tissues and 20 cases of non-cancer patients. Results The deletion of 4977bp fragment of mitochondrial DNA was detected in normal lung tissues of lung cancer tissues, adjacent normal lung tissues and non-cancer patients. Twenty cases (54.1%) of 37 lung cancer specimens detected 4977bp deletions, and thirty-six corresponding paratumoral lung tissues (22.59%) were missing, of which 8 cases were not shown in lung cancer tissues but were adjacent to the cancer. Tissues were detected as missing, and 6 of the 20 non-cancerous patients with normal lung tissue also had a 4977 bp deletion. The 4977bp deletion of mitochondrial DNA is associated with age and smoking factors. Conclusion The 4977bp deletion of mitochondrial DNA is not a lung cancer-specific mutation and is unlikely to play an important role in the process of carcinogenesis. It may be only a reflection of environmental and genetic factors in the process of tumor development.