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目的:探讨乙型肝炎病毒(hepatitisBvirus,HBV)转录体的检测在母婴传播中的意义.方法:自HBV表面抗原(HBsAg)阳性母亲及其新生儿血清中提取核酸,经PCR及RTPCR扩增HBVDNA,全长型RNA(FulllengthRNA,fRNA)和顿挫型RNA(TruncatedRNA,trRNA),琼脂糖凝胶电泳显示产物.结果:HBeAg(+)/HBeAg(-)母亲血样病毒DNA,fRNA和trRNA的阳性率分别为75%,65%,70%和37.5%,20.8%,58.3%,两组间前两项指标相差显著.44例新生儿血样HBsAg,病毒DNA和fRNA阳性率分别为9.1%,4.5%和6.8%,显著低于其母亲;儿血中trRNA阳性率达52.3%,与其母亲trRNA的存在有一定关联.15例乙肝五项检测仅显示HBsAb阳性的新生儿血样中均未见到病毒DNA和fRNA,但其中11例(73.3%)可检测到trRNA.结论:与HBsAg、病毒DNA及fRNA相比,trRNA可能是HBV母婴传播过程中早期出现的血清学指标,这一指标的应用可能有助于更精确的确定新生儿HBV感染的状态.
Objective: To investigate the significance of hepatitis B virus (HBV) transcript detection in mother-to-infant transmission.Methods: Nucleic acid was extracted from the serum of HBsAg positive mothers and their newborns, amplified by PCR and RTPCR HBVDNA, fulllength RNA (fRNA) and truncated RNA (trRNA) were detected by agarose gel electrophoresis.Results: The positive rates of viral DNA, fRNA and trRNA in blood samples of HBeAg (+) / HBeAg The positive rates of HBsAg, viral DNA and fRNA in 44 neonates were 9.1%, 4.5%, respectively % And 6.8%, respectively, which was significantly lower than that of their mothers.The positive rate of trRNA in children blood was 52.3%, which was correlated with the presence of trRNA in their mothers.15 Five cases of hepatitis B only showed no virus in HBsAb-positive neonates DNA and fRNA, but trRNA was detected in 11 (73.3%) of them.Conclusion: Compared with HBsAg, viral DNA and fRNA, trRNA may be an early serological marker in the process of mother-to-child transmission of HBV, and the application of this indicator May help to more accurately determine the status of neonatal HBV infection.