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选取1日龄健康无免疫的樱桃谷肉鸭600只,随机分成A、B、C、D 4组,每组150只。B、C和D组在7日龄分别免疫H5N1亚型禽流感灭活疫苗(Re-6+Re-8)0.5、0.6和0.8 m L/只。A组为非免疫对照组。采用HI方法检测其母源抗体和免疫抗体水平,根据母源抗体和免疫抗体消长规律探讨樱桃谷鸭H5NI亚型禽流感灭活疫苗(Re-6+Re-8)的合理免疫程序。结果表明:樱桃谷鸭Re-6与Re-8母源抗体效价和抗体合格率在1日龄最高,分别为7.8log2和100%、7.6log2和100%,14日龄下降至3.93 log2和53.3%、3.78 log2和50.0%,21日龄之后几乎没有保护力;3个剂量试验组免疫抗体水平和抗体合格率在统计学上差异不显著(P>0.05)。建议樱桃谷肉鸭在7日龄免疫,剂量为0.5m L/只。
Sixty one-day-old healthy and non-immune cherry Valley ducks were selected and randomly divided into A, B, C and D 4 groups, 150 in each group. Groups B, C and D were immunized with 0.5, 0.6 and 0.8 m L / animal of H5N1 subtype avian influenza inactivated vaccine (Re-6 + Re-8) at 7 days of age. Group A is non-immune control group. The HI antibody was used to detect the level of maternal antibodies and immune antibodies. The rational immunization program of H5NI subtype avian influenza inactivated vaccine against Cherry Valley Duck (Re-6 + Re-8) was explored according to the growth and decline of maternal antibodies and immune antibodies. The results showed that the titers and antibody passing rates of Re-6 and Re-8 maternal antibodies in Cherry Valley Duck were the highest at 1 day, 7.8 log 2 and 100%, 7.6 log 2 and 100%, respectively, and decreased from 14 days to 3.93 log 2 and 53.3%, 3.78 log2 and 50.0%, respectively. There was almost no protective effect after 21 days of age. There was no significant difference in antibody level and antibody passing rate between the three dose groups (P> 0.05). Cherry Valley ducks were recommended to be immunized at 7 days of age at a dose of 0.5 m L / bird.