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目的评价鼠疫菌检测技术在鼠疫实际监测工作的应用效果。方法分别用细菌培养、反向血凝、金标、PCR和ELISA方法检测染疫动物脏器材料F1抗原,比较了不同方法在实际监测工作中的差异。结果细菌培养、ELISA、R IHA、PCR、金标阳性检出率分别为4.68(、8.19(、15.79(、15.20(、14.62(;以标准值为对照,敏感度和特异度分别为细菌培养:敏感度36.4%、特异度100%;ELISA敏感度63.6%、特异度100%;R IHA敏感度72.7%、特异度92.6%;PCR敏感度95.5%、特异度96.6%;金标敏感度81.8%、特异度95.3%。结论细菌培养在实际应用中,受到材料腐败程度影响较大,但能培养出鼠疫菌。反向血凝在应用中以其方便、迅速且成本较低,在大范围的疫源监测中起到重要作用。胶体金法在应用中简单、准确、便捷,适用于现场操作。PCR技术在非典型鼠疫菌的检测中意义重大[1]。ELISA技术方便、快捷、特异性强,但对材料要求较高。
Objective To evaluate the application effect of Yersinia pestis detection technique in the actual monitoring of plague. Methods Bacterial culture, reverse hemagglutination, gold standard, PCR and ELISA were used to detect the F1 antigen of visceral organs of infected animals. The differences of different methods in actual monitoring were compared. Results The positive rates of bacterial culture, ELISA, R IHA, PCR and gold standard were 4.68 (, 8.19 (, 15.79, 15.20, 14.62, respectively; the standard value was control and the sensitivity and specificity were bacterial culture: Sensitivity 36.4%, specificity 100%; ELISA sensitivity 63.6%, specificity 100%; R IHA sensitivity 72.7%, specificity 92.6%; PCR sensitivity 95.5%, specificity 96.6%; gold standard sensitivity 81.8% , Specificity was 95.3% .Conclusion Bacterial culture in practical applications, influenced by the degree of material corruption, but can produce Yersinia pestis.Reverse blood coagulation in the application of its convenient, rapid and low cost, in a wide range of Epidemiological monitoring plays an important role in the application of colloidal gold method in the application of simple, accurate and convenient for field operations. PCR technology in the detection of atypical Y. pestis significant [1] ELISA technology is convenient, fast and specific Strong, but demanding materials.