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目的探讨采用自主研发的似然比率计算器进行ITO法亲缘关系鉴定的可行性。方法针对GoldeneyeTMDNA ID System 20A系统的19个常染色体STR基因座分型数据,使用自主研发的似然比率计算器,人工模拟100 000对无关个体及100 000个家系的父子、同胞、半同胞、一、二代堂表兄弟,应用ITO法,自动分析计算亲权指数(PI)和亲缘关系概率(RCP)。结果父子和无关个体关系概率界值下无重叠,具有显著差异。同胞关系概率大于99.99%时,同胞占66.48%,无关个体占0%,具有显著差异;概率小于99.99%时,同胞个体和无关个体有部分重叠,具有一定差异。半同胞关系概率大于99.99%时,半同胞占1.52%,无关个体占0%,具有显著差异;概率小于99.99%时,两组有部分重叠,具有一定差异。一、二代堂表兄弟关系概率为10%～90%范围内时,无关个体分别占90%,99.98%,无法推断。结论本文方法可用于推断父子关系、同胞关系、半同胞关系。
Objective To explore the feasibility of identifying the genetic relationship of ITO by the self-developed Likelihood Ratio Calculator. Methods According to the 19 autosomal STR loci typing data of GoldeneyeTM DNA ID System 20A system, the self-developed likelihood ratio calculator was used to simulate 100 000 unrelated individuals and their father-son, sibling, half-sibling, , II generation cousins, the application of ITO method, automatic analysis and calculation of the parental index (PI) and genetic relationship probability (RCP). Results There was no significant overlap between the probability of father-son and unrelated individuals in the relationship probability. When the probability of sibling relationship is greater than 99.99%, sibs have 66.48% of the siblings and 0% of the unrelated individuals have significant differences. When the probability is less than 99.99%, there are some differences between siblings and unrelated individuals. Semi-siblings with a probability of more than 99.99%, half siblings accounted for 1.52%, unrelated individuals accounted for 0%, with significant differences; probability of less than 99.99%, the two groups have some overlap, with some differences. First, the second generation cousin relationship probability of 10% to 90% of the range, unrelated individuals accounted for 90%, 99.98%, can not be inferred. Conclusion The method of this paper can be used to infer the relationship between father and son, sibship, half-sibship.