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目的分析PM_(2.5)空气污染物致大鼠呼吸系统炎症损伤过程中肺部炎症细胞因子表达变化。方法90只Wistar大鼠随机分成12组,每组6~8只。染尘染毒组大鼠经气管注入PM_(2.5)混悬液染尘,第2天动式染毒,对照组经气管注入生理盐水并吸入正常空气;ELISA法检测大鼠肺泡灌洗液(BALF)中炎症细胞因子含量,实时荧光定量PCR技术检测大鼠肺组织中炎症细胞因子mRNA表达。结果与对照组比较,染尘染毒1、7、30 d染尘染毒组大鼠BALF中细胞因子白细胞介素-4(IL-4)、IL-6、肿瘤坏死因子-α(TNF-α)含量及大鼠肺组织中IL-4、IL-6、TNF-αm RNA表达量均表现为先升高后下降趋势,而γ干扰素(IFN-γ)变化不明显,但呈持续下降趋势。结论在PM_(2.5)空气污染物致大鼠呼吸系统炎症损伤过程中,体液免疫应答占主导地位,Th1/Th2平衡失调,PM_(2.5)空气污染物不能刺激Th1保护性免疫反应;巨噬细胞清除PM_(2.5)空气污染物动力不足。
Objective To analyze the changes of inflammatory cytokines in the lung during PM 2.5 air pollutants induced respiratory inflammation in rats. Methods Ninety Wistar rats were randomly divided into 12 groups with 6-8 rats in each group. Rats in the dust-exposed group were exposed to PM 2.5 suspension through the endotracheal tube and were exposed to the air for 2 days. The control group was injected with normal saline through the trachea and inhaled into the normal air. BALF), and the mRNA expression of inflammatory cytokines in lung tissue of rats were detected by real-time fluorescence quantitative PCR. Results Compared with the control group, the levels of IL-4, IL-6 and TNF-α in the BALF were significantly increased in the 1, α) and the expression levels of IL-4, IL-6 and TNF-αmRNA in lung tissue of rats were increased firstly and then decreased, while the changes of IFN-γ were not obvious, but kept decreasing trend. Conclusions The humoral immune response predominates in respiratory system inflammatory injury induced by PM 2.5 air pollutants, and the Th1 / Th2 balance is imbalanced. PM 2.5 air pollutants can not stimulate Th1 protective immune response. Macrophages Remove PM_ (2.5) Air pollutants are not motivated.